rabeprazole sodium  (Sartorius AG)

Journal: Cells, Tissues, Organs

Article Title: Downregulation of Endo-Beta-N-Acetylglucosaminidase in Caenorhabditis elegans Improves Stress Adaptivity

doi: 10.1159/000546244

Figure Lengend Snippet: Rabeprazole sodium inhibited CeENGASE in vitro and improved wild-type worms stress adaptivity in vivo. a CeENGASE expressed in E. coli BL21 (DE3) was purified and subjected to an in vitro assay to release N-glycans on RNaseB. The products of the assay were analyzed by MALDI-TOF MS, and N2H3F1X1 (1221) was used as an internal control. The ratio of the main product (N1H7, 1378) to the internal reference (N2H3F1X1, 1211) decreased by about 50 times, indicating that Rabeprazole sodium inhibited the enzymatic activity of CeENGASE. b The survival rate of wild-type worms (on plate supplied with 150 μL of Rabeprazole sodium [1 m m ]) after 7-h heat treatment. The survival rate of Rabeprazole sodium group was 26.48% more than the control group (control vs. Rabeprazole sodium: 10.12% vs. 36.60%).

Article Snippet: In brief, a 200-μL reaction system was incubated at 21°C for 24 h, which contained 50 m m sodium acetate reaction buffer (pH 5.5), 100 μg RNaseB (Sigma), 100 μg CeENGASE, and 50 μM Rabeprazole sodium (Topscience) [ ].

Techniques: In Vitro, In Vivo, Purification, Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, Control, Activity Assay, Mass Spectrometry

Journal: Cells, Tissues, Organs

Article Title: Downregulation of Endo-Beta-N-Acetylglucosaminidase in Caenorhabditis elegans Improves Stress Adaptivity

doi: 10.1159/000546244

Figure Lengend Snippet: Rabeprazole sodium inhibited CeENGASE in vitro and improved wild-type worms stress adaptivity in vivo. a CeENGASE expressed in E. coli BL21 (DE3) was purified and subjected to an in vitro assay to release N-glycans on RNaseB. The products of the assay were analyzed by MALDI-TOF MS, and N2H3F1X1 (1221) was used as an internal control. The ratio of the main product (N1H7, 1378) to the internal reference (N2H3F1X1, 1211) decreased by about 50 times, indicating that Rabeprazole sodium inhibited the enzymatic activity of CeENGASE. b The survival rate of wild-type worms (on plate supplied with 150 μL of Rabeprazole sodium [1 m m ]) after 7-h heat treatment. The survival rate of Rabeprazole sodium group was 26.48% more than the control group (control vs. Rabeprazole sodium: 10.12% vs. 36.60%).

Article Snippet: In brief, a 200-μL reaction system was incubated at 21°C for 24 h, which contained 50 m m sodium acetate reaction buffer (pH 5.5), 100 μg RNaseB (Sigma), 100 μg CeENGASE, and 50 μM Rabeprazole sodium (Topscience) [ ].

Techniques: In Vitro, In Vivo, Purification, Control, Activity Assay

Journal: Bio-protocol

Article Title: An Assay System for Plate-based Detection of Endogenous Peptide: N -glycanase/NGLY1 Activity Using A Fluorescence-based Probe

doi: 10.21769/BioProtoc.5151

Figure Lengend Snippet:

Article Snippet: Rabeprazole sodium salt (Tokyo Chemical Industry Co. Ltd., catalog number: R0115) 10.

Techniques: Concentration Assay, Protease Inhibitor

Journal: Bio-protocol

Article Title: An Assay System for Plate-based Detection of Endogenous Peptide: N -glycanase/NGLY1 Activity Using A Fluorescence-based Probe

doi: 10.21769/BioProtoc.5151

Figure Lengend Snippet:

Article Snippet: Rabeprazole sodium salt (Tokyo Chemical Industry Co. Ltd., catalog number: R0115) 10.

Techniques: Concentration Assay, Protease Inhibitor

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Contribution of human organic anion transporter 3-mediated transport of a major linezolid metabolite, PNU-142586, in linezolid-induced thrombocytopenia.

doi: 10.1016/j.biopha.2024.116801

Figure Lengend Snippet: Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), rabeprazole (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.

Article Snippet: Linezolid and rabeprazole were sourced from LKT Laboratories, Inc. (St. Paul, MN).

Techniques: Inhibition, Incubation